Who we are

We are a team of University professors and researchers, with more than 25 years of experience in the use of Assisted Reproductive Technologies (ARTs) in different species.

We are continuosly using our own products in research activities to gain knowledge about better embryo environment and improving the final quality of in vitro-derived embryos.

We have learnt, from our research, that there is no better environment for the production of healthy offspring than the physiological one. Consequently,  our goal is to offer the most natural products for the ARTs of the different mammalian species.

Where are we?

We work in the Department of Physiology at the University of Murcia and the technical plant of EmbryoCloud is located very close, at the Center for Multidisciplinar Research in Bioscience (PLEIADES building) in the Campus de Espinardo, Universidad de Murcia, Murcia 30100, Spain.

Our philosophy

There is no better environment for the production of healthy offspring than the physiological one.

Goals & values
  1. To offer the most natural products for the ARTs of the different mammalian species.
  2. To improve embryo quality throughout the use of our NaturARTs product.
Our experience
We have a number of publications and projects in the field.

GARCÍA-MARTÍNEZ S, SÁNCHEZ-HURTADO MA, GUTIÉRREZ H, SÁNCHEZ-MARGALLO FM, ROMAR R, LATORRE R, COY P, LÓPEZ-ALBORS O.  Mimicking physiological O2 tension in the female reproductive tract improves Assisted Reproduction outcomes in pig. Molecular Human Reproduction 2018, 24(5): 260-270. DOI: 10.1093/molehr/gay008.

CANOVAS S, IVANOVA E, ROMAR R, GARCÍA-MARTÍNEZ S, SORIANO-ÚBEDA C, GARCÍA-VÁZQUEZ FA, SAADEH H, ANDREWS S, KELSEY G, COY P. DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids. eLife. 2017;6:e23670. DOI: http://dx.doi.org/10.7554/eLife.23670

LOPERA-VASQUEZ R, HAMDI M, MAILLO M, LLOREDA M, COY P, GUTIERREZ-ADAN A, BERMEJO-ALVAREZ P, RIZOS D. Effect of bovine oviductal fluid on development and quality of bovine embryos produced in vitro. Reproduction, Fertility and Development. 29(3) 621-629. 2017. DOI: 10.1071/RD15238.

ROMAR R, FUNAHASHI H, COY P. In vitro fertilisation in pigs: new molecules and protocols to consider in the forthcoming years. Review. Theriogenology 2016 Jan 1;85(1):125-34. doi: 10.1016/j.theriogenology.2015.07.017.

COY P, YANAGIMACHI R. Common and species-specific roles of oviductal proteins in mammalian fertilization and embryo development. BioScience, 65 (10): 973-984. doi: 10.1093/biosci/biv119

AVILÉS M, RIZOS D, COY P.  The oviduct, a key organ for the success of early reproductive events. Animal Frontiers 5:25-31, 2015. doi:10.2527/af.2015-0005

BALLESTER L, ROMERO-AGUIRREGOMEZCORTA J, SORIANO-ÚBEDA C, MATÁS C, ROMAR R, COY P. Timing of oviductal fluid collection, steroid concentrations and sperm preservation method affect in vitro fertilization efficiency. Fertility and Sterility. Fertil Steril. 2014. 102(6): 1762-1768.e1. doi: 10.1016/j.fertnstert.2014.08.009.

COY P, CANOVAS S, MONDÉJAR I, SAAVEDRA MD, ROMAR R, GRULLON L, MATÁS C, AVILÉS M.  Oviduct-specific glycoprotein and heparin modulate sperm-zona pellucida interaction during fertilization and contribute to the control of polyspermy. Proc Natl Acad Sci USA. 2008. 105(41):15809-14. doi: 10.1073/pnas.0804422105.

MONDÉJAR I, AVILÉS M, COY P. The human is an exception to the evolutionarily-conserved phenomenon of pre-fertilization zona pellucida resistance to proteolysis induced by oviductal fluid. Human Reproduction 2013 Mar;28(3):718-28. doi: 10.1093/humrep/des423.

MONDÉJAR I, MARTÍNEZ-MARTINEZ I, AVILÉS M, COY P. Identification of Potential Oviductal Factors Responsible of the Zona Pellucida Hardening and Monospermy During Fertilization in Mammals. Biol Reprod. 2013 Sep 27;89(3):67.doi: 10.1095/biolreprod.113.111385

COY P, GARCÍA-VAZQUEZ FA, VISCONTI P, AVILES M. Roles of the oviduct in mammalian fertilization. Reproduction (2012) 144 649–660. DOI: 10.1530/REP-12-0279

MONDÉJAR I, ACUÑA S, IZQUIERDO RICO MJ, COY P, AVILÉS M. The oviduct: functional genomic and proteomic approach. Reproduction in Domestic Animals 2012 Jun;47 Suppl 3:22-9.).

HUNTER RHF, COY P, GADEA J, RATH D. Consideration of viscosity in the preliminaries to mammalian fertilisation. Journal of Assisted Reproduction and Genetics. J Assist Reprod Genet. 2011 Mar;28(3):191-7.

COY P, AVILES M. What controls polyspermy in mammals, the oviduct or the oocyte? Biol Rev Camb Philos Soc. 2010; 85(3):593-605.

COY P, LLOYD R, ROMAR R, SATAKE N, MATAS C, GADEA J, HOLT WV. Effects of porcine pre-ovulatory oviductal fluid on boar sperm function. Theriogenology. 74(4):632-642. 2010.

AVILES M, GUTIERREZ-ADAN A, COY P. Oviductal secretions: Will they be key factors for the future ARTs?. Molecular Human Reproduction. 2010 16 (12): 896-906.

LLOYD RE, ROMAR R, MATAS C, GUTIÉRREZ-ADÁN A, HOLT WV, COY P. Effects of oviductal fluid on the development, quality and gene expression of porcine blastocyst produced in vitro. Reproduction. 2009 Apr;137(4):679-87[/vc_column_text]

Projects and Contracts


From assisted reproductive technologies onwards: their impact on preimplantation epigenetic reprogramming and their use in the generation of CRISPR-Cas9 transgenic animals.  
Principal investigator: Drs. Pilar Coy and Joaquín Gadea.  Agency of Science and Technology of Murcia. Séneca foundation. Project 20040/GERM/16.

Obtaining healthy animals using assisted reproduction techniques based on physiological conditions
Principal investigator: Drs. Pilar Coy and Joaquín Gadea.  Spanish Ministry of Economy and Competitiveness. Project AGL2015-66341-R.

Description of new physiological pathways participating in mammalian fertilization.
Principal investigator: Dr. Pilar Coy. Spanish Ministry of Economy and Competitiveness.  Project AGL 2012-40180-C03-01.

Generation of new knowledge on the biological mechanism of fertilization and application of the results in the improvement of the in vitro fertilization technique in mammals.
Principal investigator: Dr. Pilar Coy.  Spanish Ministry of Science and Education and FEDER. Project AGL 2009-12512-C02-01.

Genomic study of the porcine oviduct in relation with interactions produced during fertilization.
Principal investigator: Dr. Pilar Coy. Fundación Séneca (Center for Research Coordination), Comunidad Autónoma Región de Murcia.  Project 11996/PI/09.

Role of oviductal and acrosomal glycosidases in pig sperm-oocyte interaction.
Principal investigator: Dr. Pilar Coy. Fundación Séneca (Center for Research Coordination), Comunidad Autónoma Región de Murcia. Project 03018/PI/05.