{"id":7108,"date":"2024-12-16T14:18:08","date_gmt":"2024-12-16T14:18:08","guid":{"rendered":"https:\/\/embryocloud.com\/?page_id=7108"},"modified":"2025-06-04T12:22:29","modified_gmt":"2025-06-04T12:22:29","slug":"frequently-asked-questions","status":"publish","type":"page","link":"https:\/\/embryocloud.com\/frequently-asked-questions\/","title":{"rendered":"Preguntas Frecuentes"},"content":{"rendered":"
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Preguntas Frecuentes<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Preguntas frecuentes sobre los medios de EmbryoCloud para la producci\u00f3n de embriones in vitro<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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1. \u00bfCu\u00e1les son las ventajas de los medios de cultivo en formato seco? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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Los costos de env\u00edo son m\u00e1s bajos porque no se requiere refrigeraci\u00f3n durante el transporte. Los medios de cultivo en formato seco tienen una vida \u00fatil mucho m\u00e1s larga en comparaci\u00f3n con sus equivalentes l\u00edquidos, permitiendo su almacenamiento durante al menos 1 a\u00f1o a 4\u00b0C. Tambi\u00e9n pueden almacenarse a temperatura ambiente durante al menos 5 meses.<\/p>

Adem\u00e1s, la ausencia de agua hace que los medios de cultivo sean menos susceptibles a la contaminaci\u00f3n microbiol\u00f3gica.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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2. \u00bfC\u00f3mo puedo reconstituir los medios de cultivo en formato seco a su forma l\u00edquida? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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EmbryoCloud proporciona la cantidad necesaria de agua ultrapura para cada medio de cultivo en formato seco. Simplemente a\u00f1ade la cantidad indicada de agua al vial que contiene el polvo.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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3. \u00bfCu\u00e1nto tiempo debo esperar antes de usar el medio de cultivo despu\u00e9s de a\u00f1adir el agua ultrapura? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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EmbryoCloud recomienda disolver el polvo durante al menos 6 horas a una temperatura c\u00e1lida, idealmente durante la noche. Alternativamente, el polvo puede disolverse a 4\u00baC en el refrigerador por un m\u00ednimo de 8 horas.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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4. \u00bfQu\u00e9 debo hacer si el medio de cultivo no se ha disuelto correctamente? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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EmbryoCloud recomienda agitar la soluci\u00f3n con un vortex durante 10 segundos y asegurarse de que no queden part\u00edculas en suspensi\u00f3n.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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5. \u00bfEst\u00e1 el medio de cultivo listo para usar una vez disuelto? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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S\u00ed, una vez reconstituido, el medio est\u00e1 listo para usar. Sin embargo, EmbryoCloud recomienda filtrar el medio antes de su uso con un filtro de 0,22 \u00b5m de tama\u00f1o de poro. Adem\u00e1s, los medios de maduraci\u00f3n, fertilizaci\u00f3n y cultivo de embriones deben ser equilibrados durante al menos 2 horas antes de su uso en una atm\u00f3sfera de 5% de CO\u2082.<\/p>

\u00a0<\/h3>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t
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6. \u00bfPuedo continuar utilizando mi protocolo habitual para la producci\u00f3n in vitro de embriones? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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S\u00ed, absolutamente. Una vez reconstituido, el medio se puede utilizar como cualquier otro medio de cultivo l\u00edquido.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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7. \u00bfDebo utilizar todo el volumen del medio de cultivo o puedo guardar algo para m\u00e1s tarde? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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Utilice solo la cantidad que necesite. El medio restante se puede almacenar a 4\u00b0C, bien sellado, durante hasta 1 mes sin comprometer los resultados.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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8. \u00bfQu\u00e9 controles de calidad se realizan en los medios de cultivo en formato seco? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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Cada lote de medio de cultivo se prueba para pH, osmolaridad, endotoxinas, contaminaci\u00f3n bacteriana y f\u00fangica. Adem\u00e1s, se realizan ensayos embrionarios bovinos.<\/span><\/p>

Se realizan ensayos embrionarios bovinos (BEA) o ensayos embrionarios porcinos (PEA). Estos consisten en la producci\u00f3n de embriones in vitro a partir de ovarios de matadero y semen congelado.<\/span><\/p>

Los ensayos embrionarios se eval\u00faan en funci\u00f3n de la tasa de segmentaci\u00f3n (porcentaje de cigotos divididos 24 horas despu\u00e9s de la fertilizaci\u00f3n) y la tasa de blastocistos en los d\u00edas 7 y 8 posteriores a la fertilizaci\u00f3n (porcentaje de blastocistos en relaci\u00f3n con el n\u00famero total de oocitos).<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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9. \u00bfQu\u00e9 resultados se pueden esperar para la producci\u00f3n de embriones con estos medios? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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En los ensayos embrionarios de EmbryoCloud utilizando ovarios de matadero y semen congelado, las tasas de segmentaci\u00f3n superan el 70% y las tasas de blastocistos superan el 20% en el d\u00eda 7 posterior a la fertilizaci\u00f3n, tanto para especies bovinas como porcinas.<\/span><\/p>

Estos son nuestros umbrales de control de calidad. Sin embargo, debido a la variabilidad biol\u00f3gica (por ejemplo, la edad del donante, el origen de los ovarios de matadero, la calidad del semen), los resultados pueden variar, alcanzando hasta un 90% de segmentaci\u00f3n y un 45% de tasas de blastocistos para oocitos bovinos y porcinos.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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10. \u00bfPara qu\u00e9 especies animales est\u00e1n dise\u00f1ados estos medios? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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EmbryoCloud ofrece una gama completa de medios para la producci\u00f3n in vitro de embriones tanto en especies bovinas como porcinas.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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11. \u00bfLos medios de cultivo contienen fluidos reproductivos? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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S\u00ed, los medios de maduraci\u00f3n in vitro en formato seco de la l\u00ednea NaturARTS incluyen fluido folicular, lo que hace que la composici\u00f3n de los medios de cultivo sea m\u00e1s similar a las condiciones fisiol\u00f3gicas que los embriones experimentan in vivo. Tanto en especies bovinas como porcinas, los resultados muestran una mejor producci\u00f3n de embriones cuando los oocitos se maduran con medios que contienen fluido folicular.<\/span><\/p>

Los fluidos reproductivos se prueban para detectar varios pat\u00f3genos antes de ser utilizados como suplementos en los medios, y solo se utilizan fluidos libres de virus.<\/span><\/p>

  • Virus bovinos: IBR, BVD y lengua azul.<\/span><\/li>
  • Virus porcinos: Circovirus tipo 2, PRRS 1, PRRS 2, parvovirus. Adem\u00e1s, est\u00e1n libres de otras enfermedades de notificaci\u00f3n obligatoria:<\/span><\/li>
  • Bovino: Brucelosis, Tuberculosis.<\/span><\/li>
  • Porcino: Fiebre Africana porcina, Fiebre cl\u00e1sica porcina.<\/span><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t
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    12. \u00bfExisten alternativas a los medios que contienen fluidos reproductivos? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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    S\u00ed, existen medios qu\u00edmicamente definidos para la maduraci\u00f3n in vitro de oocitos, donde el fluido folicular es reemplazado por BSA. Sin embargo, nuestra recomendaci\u00f3n es utilizar la l\u00ednea NaturARTS para obtener los mejores resultados.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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    13. \u00bfExisten publicaciones que utilicen los medios o fluidos reproductivos de EmbryoCloud? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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    S\u00ed, est\u00e1n disponibles en nuestro sitio web (https:\/\/embryocloud.com\/) o pueden enviarse por correo electr\u00f3nico.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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    \n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

    Frequently Asked Questions FAQs About EmbryoCloud\u2019s Media for In Vitro Embryo Production 1. What are the advantages of the dry culture media? Shipping costs are lower because refrigeration is not required during transport. Dryform cobfersculture media with a have a much longer shelf life compared to their liquid counterparts, allowing storage for at least 1 […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_surecart_dashboard_logo_width":"180px","_surecart_dashboard_show_logo":true,"_surecart_dashboard_navigation_orders":true,"_surecart_dashboard_navigation_invoices":true,"_surecart_dashboard_navigation_subscriptions":true,"_surecart_dashboard_navigation_downloads":true,"_surecart_dashboard_navigation_billing":true,"_surecart_dashboard_navigation_account":true,"_uag_custom_page_level_css":"","site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"full-width-container","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-7108","page","type-page","status-publish","hentry"],"yoast_head":"\nFrequently Asked Questions - EmbryoCloud<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/embryocloud.com\/es\/frequently-asked-questions\/?currency=eur\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Frequently Asked Questions - EmbryoCloud\" \/>\n<meta property=\"og:description\" content=\"Frequently Asked Questions FAQs About EmbryoCloud\u2019s Media for In Vitro Embryo Production 1. What are the advantages of the dry culture media? Shipping costs are lower because refrigeration is not required during transport. 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